Distribution of pg-m/versican variants in human tissues andde novo expression of isoform v3 upon endothelial cell activation, migration, and neoangiogenesis in vitro *

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  • who: Sabrina Cattaruzza from the Antibodies-A panel of anti-versican mAbs was produced according to standard procedures by immunizing Balb/c female mice with isolated bovine aorta versicans and screening the hybridomas against the immunogen and related antigensSpecificity of these mAbs has partially been described elsewhere (3, ) and further details about their reactivities were determined here by enzyme-linked immunosorbent assay, dot-blot assays, and immunoblotting on intact and enzyme-digested PGs, including versicans from various bovine and human sources, aggrecans from cartilage tissues of various animal species, fibromodulin, decorin, and biglycan. An antiserum produced against . . .

     

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