HIGHLIGHTS
- who: madub from the Ghent University have published the article: And…cut! Identifying chromatin features affecting CRISPR-Cas9 activity in plants 2, in the Journal: (JOURNAL)
- what: In the work presented by Weiss et_al, , a genome-wide search of the Arabidopsis genome identified over 7 million putative CRISPR target sites (20 nucleotides followed by NGG, the PAM recognition site).
SUMMARY
To eliminate the effect that the gRNA sequence would have on Cas9 efficacy, the authors aimed to target identical short sequences within their specific, natural genomic loci. Subsequently, they filtered the . . .
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