Laminin receptor involvement in the anti-angiogenic activity of pigment epithelium-derived factor*⒦

HIGHLIGHTS

• who: Adrien Bernard from the Co-immunoprecipitation-We incubated the protein mixtures at , °C for , h with , ␮g of anti-PEDF antibody (Chemicon) on a mixing rotorWe added agarose A/G protein (, ␮l) and incubated the samples overnight. The next day, we centrifuged the samples at , ⫻ g for , min. We removed the supernatant, leaving a pellet of beads. We washed the pellets four times by centrifuge (, ⫻ g, min) with radioimmune precipitation assay buffer (, ml) (1⫻ PBS, pH, .4, % Nonidet, .5% sodium deoxycholate, .1% SDS), adding Laemmli buffer after the last wash. We performed Western blot analysis with . . .
  

   

  

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