HIGHLIGHTS
- who: Pristionchus pacificus genetic and collaborators from the University of Arlington, Arlington, TX, USA have published the research work: Pristionchus pacificus genetic protocols, in the Journal: (JOURNAL)
SUMMARY
Add 250 µl of E. coli OP50 to each 6 cm diameter plate. Add 3 ml of 0.1 M MgSO4] 1.8 ml cryotube vials Grow worms on about 10 NGM plates until one day beyond the time the plates are just cleared of bacteria. Heat-shock worms on the plates at 30°C for 2-3 hours. Wash each plate with 2 ml . . .
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