Evolutionary origin of vertebrate oct4/pou5 functions in supporting pluripotency

HIGHLIGHTS

• who: Woranop Sukparangsi from the Mouse ESC culture Mouse ESCs were routinely cultured as described by ref38. Briefly, complete mouse ESC medium was composed of Glasgow Minimum Essential Medium (GMEM) containing, ., mM non-essential amino acids, mM, glutamine, ., mM sodium pyruvate, ., mM u03b2-mercaptoethanol, % Fetal Bovine Serum (FBS) and murine LIF (homemade). The flasks/dishes (Corning) for ESC culture were coated with, .1% gelatin in PBS. Reagents used for, iL (N, u03bcM PD0325901, u03bcM , and LIF on gelatin), Rosette (N, u03bcM IWP2, u03bcM , and LIF on gelatin have published the research: Evolutionary origin of vertebrate OCT4/POU5 . . .
  

   

  

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