Membrane-anchored human rab gtpases directly mediate membrane tethering in vitro

HIGHLIGHTS

• who: Naoki Tamura and collaborators from the (UNIVERSITY) have published the Article: Membrane-anchored human Rab GTPases directly mediate membrane tethering in vitro, in the Journal: (JOURNAL) of 30/06/2014
• how: Next to test whether Rab-mediated liposome aggregation can be competitively blocked by addition of untagged Rab proteins which have no C-terminal His12 tag for membrane attachment but may be able to associate with membrane-anchored Rab-His12 proteins the authors employed the turbidity assays in the presence of both Rab5a-His12 and untagged Rab5a (Fig 5E).
• future: Reconstitution studies . . .
  

   

  

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