Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases nedd4 and nedd4-2*

HIGHLIGHTS

• who: Andrew B. Fotia from the Bacterial Expression Plasmids-The Nav channel carboxyl-terminal region-glutathione, transferase (GST) fusion constructs were generated as followsThe carboxyl termini were PCR-amplified from mouse brain cDNA (Nav1.1, Nav1.2, Nav1.3, and Nav1.6), mouse day , embryo cDNA (Nav1., and Nav1.7), and mouse dorsal root ganglion cDNA (Nav1.8) (donated by A. Stokowski) and cloned into either the BamHI or BamHI/ EcoRI sites of pGEX- TK (Amersham Biosciences). The number of carboxyl-terminal residues present in the various Nav-GST fusion proteins are as follows: Nav1.1, have . . .
  

   

  

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