Saccharomyces cerevisiae hog1 protein phosphorylation upon exposure to bacterial endotoxin*

HIGHLIGHTS

• who: Joana M. Marques from the EXPERIMENTAL PROCEDURES Strains and Growth Conditions-The Scerevisiae strains used in this study were wild-type, A (Mata ade, can, his, leu, trp1, ura3-1), provided by Professor Johan Thevelein (Katholieke University of Leuven, Belgium), and its derivative YSH , (hog1u232c:: TRP1) mutant, obtained from Professor Stefan Hohmann (32). Yeast cells were grown at , u00b0C in yeast nitrogen base (YNB) medium supplemented with the appropriate selective amino acids and bases and, % glucose. Growth was assessed by measuring the optical density at a wavelength of , nm (, nm). The cells were grown to midlog . . .
  

   

  

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