HIGHLIGHTS
- who: Han Roelofsen from the Department University of Groningen, AV Groningen, The Netherlands have published the Article: Comparison of Isotope-labeled Amino Acid Incorporation Rates (CILAIR) Provides a Quantitative Method to Study Tissue Secretomes, in the Journal: (JOURNAL)
- what: The authors assessed whether comparing 13C-labeled lysine incorporation into newly synthesized secreted proteins at a fixed culture period could serve as a reliable method to assess insulin-induced changes in protein expression. The authors show that the CILAIR method allows quantitative assessment of changes in protein secretion without the need for 100% label incorporation.
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