Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase ul97 is required for regulatory protein 14-3-3 binding and ul97 stability

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• who: Satoko Iwahori from the Halo tag pull downs and LC-MS/MS analysis Kinase was purified from HEK- T cells transfected with pFC14a-HA-, WT or pFC14a (Promega) with a , h bead incubation as previously described (5)Samples were eluted with TEV protease (Promega) and submitted to the University of Wisconsin Biotechnology Center for LC-MS/MS analysis with a Thermo Fisher Scientific Orbitrap Elite. Two independent biological replicates were analyzed. Results were analyzed using Mascot and Scaffold viewer, and peptides proteins reported had at least one identifying peptide with, % false discovery rate threshold. In Table . . .
  

   

  

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