A minimal-complexity light-sheet microscope maps network activity in 3d neuronal systems

HIGHLIGHTS

• who: Paulina M. Wysmolek from the Max Planck Institute Mu00fcnchen, Munich, Germany have published the research work: A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems, in the Journal: Scientific Reports Scientific Reports
• how: As a discriminator for filtering the authors used the difference in spatial footprint of_(almost point-like) neurons and the global large-scale background. After this preprocessing CaImAn`s motion correction algorithm was applied before temporal 3D segmentation. To test the limits of data acquisition the authors recorded beads in agarose gels for as long as 30 . . .
  

   

  

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