Shifting the ph optima of (r)-selective transaminases by protein engineering

HIGHLIGHTS

• who: Chao Xiang and colleagues from the Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald, Germany have published the research: Shifting the pH Optima of (R)-Selective Transaminases by Protein Engineering, in the Journal: (JOURNAL)
• what: According to this analysis, the authors propose the following hypothesis the E49Q substitution destabilizes the positively charged form of the imidazole group of H53.
• how: The authors compared the 3D structures of ATA-Afu (PDB 4CHI) ATA-Gze (homology modeling by YASARA 17.1.28 ) and ATA-Ate (PDB 4CE5) but all . . .
  

   

  

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