Establishment of indirect elisa method for salmonella antibody detection from ducks based on pagn protein

HIGHLIGHTS

  • who: Shaopeng Hou from the (UNIVERSITY) have published the research work: Establishment of indirect ELISA method for Salmonella antibody detection from ducks based on PagN protein, in the Journal: (JOURNAL)
  • what: In this study, the complete DNA sequence of PagN gene was obtained by PCR, and cloned into pET-32a(+) to construct successfully the recombinant prokaryotic expression plasmid pET-32a(+)-PagN. The authors obtained a fixed cut-off of 0.268 for iELISA performance by detecting 38 Salmonella negative serum samples. Compared with the iELISA method established using PagC protein , the method the authors established . . .

     

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