In-situ forming injectable gfoger-conjugated bmscs-laden hydrogels for osteochondral regeneration

HIGHLIGHTS

  • who: Mi Yeon Ha from the , Chondrogenic differentiation of BMSCs in hydrogels BMSCs were encapsulated into hydrogel formulations at a density of , u00d7 , cells/ml in, well culture plates at , u00b0C, % CO After , h of incubation, BMSCs-encapsulated hydrogels were exposed to Stem Pro chondrogenic differentiation media (Gibco) and maintained for up to , days. The medium was replaced every , days until the experiment was terminated. Biochemical assay for glycosaminoglycan (GAG) production During the chondrogenesis of BMSCs, medium was changed every , days. GAGs were quantified using the Sulfated Glycosaminoglycans Quantification kit (AMS Biotechnology, Abingdon, UK have published the . . .

     

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