HIGHLIGHTS
SUMMARY
Capillaries with a thin tip (Fig 1a) were prepared following the standard laboratory protocol to generate microinjection needles for zebrafish embryos. The authors could successfully genotype all tested embryos (n=24 for plekhh and n=10 for lak) on which tail FS and genomic extraction were performed and isolate heterozygous animals (Fig 2b and Supplementary Fig 5) for future experimental needs and/or mutant line maintenance. Even if promising in_vitro and ex vivo methods that could serve as alternative approaches to animals are being proposed57,58, abolishing in_vivo models entirely without affecting the quality . . .
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