A novel sfani-like restriction-modification system in caldicellulosiruptor extents the genetic engineering toolbox for this genus

HIGHLIGHTS

• who: Steve Swinnen and colleagues from the BluCon Biotech GmbH, Cologne, Germany, BRAIN Biotech AG, Zwingenberg, Germany Editor:, University of Illinois at have published the research: A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus, in the Journal: PLOS ONE of 11/Dec/2022
• what: In this study system was identified in Caldicellulosiruptor sp. strain BluCon085 consisting of an endonuclease and two methyltransferases that recognize the reverse-complement sequences 5`-GATGC-3` and 5u2018-GCATC-3`. The authors show that methylation of plasmid and linear DNA by . . .
  

   

  

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