A rapid method for detecting and distinguishing metallo-β-lactamase-and serine carbapenemase-producing enterobacteriales using maldi-tof ms

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• who: ufeffXiaopengufeff ufeffJingufeff from the University of Florence, Italy have published the research work: A rapid method for detecting and distinguishing metallo-u03b2-lactamase-and serine carbapenemase-producing ufeffEnterobacterialesufeff using MALDI-TOF MS, in the Journal: IPM hydrolysis assay The Enterobacteriales strains were cultivated in blood agar medium in an aerobic environment for 18-24 h at 35 u00b1 2u00b0C. Then, a 1-u03bcL loopful of cultured bacteria was collected and emulsified in 1 ml 0.45% NaCl as previously described by Burckhardt and Zimmermann (2011). IPM was added to a concentration of 0.25 mg/ml . . .
  

   

  

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