HIGHLIGHTS
SUMMARY
Accurate gene_expression analysis at the transcript level is essential to understand all aspects of plant growth and development and their responses to abiotic and biotic stress. It improves the precision of analysis of RNA-seq data for differential gene and transcript expression and differential alternative splicing and now allows analysis of differential TSS and TES usage. The high-confidence full-length transcripts in AtIso covered ca. two-thirds of genes in Arabidopsis and confirmed many of the short-read assembled transcripts while resolving assembly artifacts present in AtRTD2. AtIso was merged with short-read . . .
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