Insulin receptor activation by proinsulin preserves synapses and vision in retinitis pigmentosa

HIGHLIGHTS

• who: Alonso Su00e1nchez-Cruz from the ethanol solutions and embedded in Epoxy EMbed, resin (EMS, Electron Microscopy Sciences)Semi-thin (0., u03bcm) and ultra-thin sections were obtained using an Ultracut E ultramicrotome (Leica). Semi-thin sections were stained with Toluidine Blue and mounted with Entellan and images were obtained using a light microscope (Axio Observer, Zeiss) with, u00d7 and, u00d7 oil immersion objectives. Ultra-thin sections were contrasted with uranyl acetate and lead citrate, and analyzed at the NUCLEUS electron microscopy facility at the University of Salamanca using a Tecnai Spirit Twin , kv electron microscope with . . .
  

   

  

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