Crispr/cas9 grna activity depends on free energy changes and on the target pam context

HIGHLIGHTS

• who: Giulia I. Corsi from the University of have published the research: CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
• what: The authors focused the analysis on off-targets with no 3-nt PAM (NGG) variation to the on-target and with up to three mismatches to the gRNA, none of which should be in the four PAM-proximal nucleotides, as this could interfere with sequence composition preferences (Fig 1b). In the following, the authors focus on overlapping PAMs with up . . .
  

   

  

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