Detection of neutrophil extracellular traps in patient plasma: method development and validation in systemic lupus erythematosus and healthy donors that carry irf5 genetic risk

HIGHLIGHTS

  • who: Bharati Matta et al. from the Linku00f6ping, Sweden of Tokyo, Japan have published the article: Detection of neutrophil extracellular traps in patient plasma: method development and validation in systemic lupus erythematosus and healthy donors that carry IRF5 genetic risk, in the Journal: (JOURNAL)
  • what: The authors developed and optimized a sensitive multiplex ELISA, as well as designed a simple, new immunofluorescence technique to both visualize and quantify NETs in small amounts of patient plasma.

SUMMARY

    Experimentally, NETs can be detected in 50µl undiluted plasma samples using single anti-MPO . . .

     

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