And . . . cut! identifying chromatin features affecting crispr-cas9 activity in plants

HIGHLIGHTS

  • who: PLANT PHYSIOLOGY from the Ghent University have published the paper: And . . . cut! Identifying chromatin features affecting CRISPR-Cas9 activity in plants, in the Journal: (JOURNAL) of June/28,/2022

SUMMARY

    Identifying chromatin features affecting CRISPR-Cas9 activity in plants PLANT PHYSIOLOGY 2022: 190; 1074-1076 Chromatin features affecting CRISPR activity # REPEATS GC% EASY GENOTYPING Cas9 + H3K9ac H3K36ac H3K36me3 1 gRNA GTTTTCTCGCACTTAAGCTCTGG CHROMATIN CONTEXT 1 GUIDE RNA 7 MULTICOPY 8 TARGET SITES CRISPR SITES 8 DIVERSE CHROMATIN CONTEXTS DNA C-me H2A.W H3K9me1 H3K9me2 Arabidopsis plants. The sequence of the DNA flanking . . .

     

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