HIGHLIGHTS
SUMMARY
This work examines cysteine residues and shows evidence for their modification during cryo-EM grid preparation, which is prevented either by adding reducing_agents such as DTT, by sequestering away from the AWI, or by fast grid preparation. In the past decade, cryo-EM has become one of the major techniques for protein structure determination with rapid improvements in microscope hardware and data processing software, recently reaching atomic resolution (Nakane et_al, 2020; Yip et_al, 2020). The vast majority of proteins are thought to interact with the AWI, which for some samples may affect particle orientation . . .
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