HIGHLIGHTS
- who: Huning Jiang from the hexamer-primerBuffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double-strand cDNA was purified with magnetic beads. End reparation and, `-end single nucleotide A (adenine) addition was then performed. Finally, sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the QC step, the Agilent , Bioanaylzer and ABI StepOnePlus Real-Time PCR System were used to qualify and quantify of the sample library, after which the library products were deemed ready for sequencing via Illumina HiSeqTM, . The whole RNA . . .
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