Circularized fluorescent nanodiscs for probing protein-lipid interactions

HIGHLIGHTS

  • who: Qian Ren from the Inc have published the Article: Circularized fluorescent nanodiscs for probing protein-lipid interactions, in the Journal: (JOURNAL) of 28/Dec/2021
  • what: Using this approach the authors successfully generated the RFP-circularized MSP1D1 (Fig 1b and Supplementary Fig 2c j and k; SPY-spmcherry11-10/11-MSP1D1) which could form homogenous 12 nm nanodiscs as shown by SEC and DLS characterizations (Supplementary Fig 3).
  • how: In this manuscript the authors described the work in the development of an intensity-based fluorescent sensor for detecting membrane binding and remodeling . . .

     

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