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- who: Hongguang Bao from the Qilu Hospital, Shandong University, China have published the research work: Short Chain Fatty Acids Protect the Cognitive Function of Sepsis Associated Encephalopathy Mice via GPR43, in the Journal: Gut Microbiota Composition Analysis A total of 1,000 mg fecal pellets were collected in sterile tubes for each mouse. Microbiota DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen, USA) and applied to amplification of V3-V4 regions of 16S rDNA. PCR amplification of the bacterial 16S rRNA genes V3-V4 region was performed using the forward primer 338F (5`-ACTCCTACGGGAGGCAGCA . . .
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