Serum neutralization activity declines but memory b cells persist after cure of chronic hepatitis c

HIGHLIGHTS

• who: Akira Nishio from the Cell Lines Huh75., hepatoma cells were generated and kindly provided by Dr. Francis V. Chisari, The Scripps Research Institute, La Jolla, CA74. They were maintained in Dulbecco`s modified Eagles medium (DMEM, Corning, New York, NY) supplemented with, % fetal bovine serum (FBS, Serum Source International, Charlotte, NC), % penicillin/streptomycin, and , mM, glutamine (Mediatech, Manassas, VA). HEK T cells and Hep B cells were kindly provided by Dr. Justin Bailey (John Hopkins University School of Medicine, Baltimore, MD) and originally purchased from ATCC (catalog numbers , and HB-8084, respectively). HEK T cells were . . .
  

   

  

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