Kctd9 inhibits the wnt/β-catenin pathway by decreasing the level of β-catenin in colorectal cancer

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  • who: Hanhui Yao from the Cell lines and human tissues All cell lines were cultured in the specified media containing, % FBS and, % penicillin/streptomycin at , u00b0C in a, % , environmentHEK T, NCM460, and , cells were cultured in DMEM, SW480, and , cells in, in RPMI-1640, and LoVo cells in, K media. All cells were tested for mycoplasma contamination by RT-PCR. Alternatively, fresh human colon cancer and adjacent noncancerous tissues were obtained from patients undergoing surgical resection at the First Affiliated Hospital of the University of Science and Technology of China (USTC, Hefei, China). The experiments using fresh . . .

     

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