A comparison of strategies for generating artificial replicates in rna-seq experiments

HIGHLIGHTS

  • who: Babak Saremi from the InstitutUniversity of have published the research: A comparison of strategies for generating artificial replicates in RNA-seq experiments, in the Journal: Scientific Reports Scientific Reports
  • what: Bootstrapping sequencing reads from FASTQ-files is a helpful approach to generate artificial replicates in RNAseq experiments.
  • how: To assess the variability between the results from the two real technical replicates R1 and R2 the authors compared the raw gene-wise p values as well as the absolute log2 fold changes of all genes after differential gene_expression analysis (Fig 2). The authors . . .

     

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