Barriers to simultaneous multilocus integration in bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the crispr-cas9 system

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  • who: Jordi Ferrando from the (UNIVERSITY) have published the Article: Barriers to simultaneous multilocus integration in Bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the CRISPR-Cas9 system, in the Journal: (JOURNAL)
  • what: This approach has the disadvantage that resulting strains have low gene dosage unless multiple rounds of gene insertion are performed , until reaching expression levels comparable to that of cells carrying multiple copies of a recombinant plasmid. For the first time, this study aimed to develop both a CRISPR-Cas9 . . .

     

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