HIGHLIGHTS
SUMMARY
After several rounds of selection to increase stringency, two sFabs, termed COP-2 and COP-3, were further developed and validated by ELISA to bind to claudin-4/cCpE. Sequence alignments of COP-2 and COP-3 reveal that COP-2 and COP-3 share 98.2 and 93.3% sequence identity in their L and H chains, respectively; and that COP sequences diverge the greatest in CDR-L3, CDR-H1, and CDR-H3 (Supporting Information (SI) Fig S1). After expressing and purifying claudin-4, cCpE, CpE, and COPs, increases in molecular masses as . . .
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