HIGHLIGHTS
SUMMARY
Due to this limited capture area, it is generally recognized that more than one IVCM image needs to be acquired and analyzed to derive a representative estimate of the corneal epithelial IC density in a particular corneal region. There has not yet been a study investigating the optimal IVCM image sample size required to derive a valid estimate of corneal epithelial IC density for a particular individual. Previous corneal IVCM studies have used a variety of sample sizes, including quantifications from three, five, eight, or twelve non-overlapping images, to derive an estimate of . . .
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