Microfluidic screening and genomic mutation identification for enhancing cellulase production in pichia pastoris

HIGHLIGHTS

  • who: Huiling Yuan from the CAS Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute China have published the research: Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris, in the Journal: (JOURNAL)
  • what: In this study whole-cell directed evolution of P. pastoris employing atmospheric and room temperature plasma (ARTP) mutagenesis and droplet-based HTS was developed to improve heterogenous cellulase production.
  • how: The droplet stability and fluorescein leakage were tested in the presence of methanol in the aqueous phase and the results showed that after 48 h off . . .

     

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