Evaluation of the α-casein (csn1s1) locus as a potential target for a site-specific transgene integration

HIGHLIGHTS

  • who: A. V. Smirnov from the InstitutState University have published the article: Evaluation of the u03b1-casein (CSN1S1) locus as a potential target for a site-specific transgene integration, in the Journal: Scientific Reports Scientific Reports
  • how: The results were analyzed using QuantaSoft software (Bio-Rad).

SUMMARY

    Two types of the hGMCSF insertion were tested: a simple knock-in, in which the hGMCSF ORF was placed behind the mouse endogenous Csn1s1 start codon (Fig 1B, top), while preserving the remaining Csn1s1 sequence; a complete replacement of the Csn1s1 ORF (Exons 2 . . .

     

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