Identification of beneficial and detrimental bacteria impacting sorghum responses to drought using multi-scale and multi-system microbiome comparisons

HIGHLIGHTS

• who: Mingsheng Qi from the steel beads until processingRoot and soil samples were pulverized using a TissueLyser II (Qiagen) with cold blocks cooled in liquid nitrogen (, min grinding, Hz, times). The orientation of sample cassettes in the TissueLyser was rotated between two grindings. DNA extractions were carried out on ground root and soil samples using DNeasy PowerPlant Pro kit (Qiagen) following the manufacturer`s instruction. Bacterial S rRNA gene sequencing. The S rRNA gene Pair-End (PE) amplicon sequencing on, regions using the primers F (5u2032-GTGC CAGCMGCCGGCGGTAA-3u2032) and R (5u2032-CGACRRCCATGCANCACCT-3u2032) was performed on . . .
  

   

  

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