Functional analysis of the p-type atpases apt2-4 from cryptococcus neoformans by heterologous expression in saccharomyces cerevisiae

HIGHLIGHTS

  • who: Sarina Veit and collaborators from the Department of Molecular Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Bochum, Germany have published the research: Functional Analysis of the P-Type ATPases Apt2-4 from Cryptococcus neoformans by Heterologous Expression in Saccharomyces cerevisiae, in the Journal: (JOURNAL)
  • what: This study provides the first biochemical analysis regarding substrate preference and subunit requirements among the C. neoformans P4-ATPases Apt2-4p based on heterologous expression in the flippase-deficient S. cerevisiae strain dnf1u2206dnf2u2206drs2u2206.
  • how: Since previous studies did not show clear phenotypes for single APT24 . . .

     

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