Archaeal dna-import apparatus is homologous to bacterial conjugation machinery

HIGHLIGHTS

• who: Leticia C. Beltran from the Cryo-EM sample preparation and data collection A, u03bcL aliquot of sample containing either Pyrobaculum calidifontis, Aeropyrum pernix, or Agrobacterium tumefaciens pili was applied to a plasma cleaned (Gatan Solarus) lacey carbon grid (Ted Pella, Inc), blotted with automated blotting for , s at, % humidity and flash frozen in liquid ethane using an EM GP Plunge Freezer (Leica). The dataset used for structure determination was collected at the Molecular Electron Microscopy Core at the University of Virginia on a Titan Krios EM operated at , keV, equipped with an energy filter and , direct . . .
  

   

  

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