The force required to remove tubulin from the microtubule lattice by pulling on its α-tubulin c-terminal tail

HIGHLIGHTS

• who: Yin-Wei Kuo from the Yale University have published the article: The force required to remove tubulin from the microtubule lattice by pulling on its u03b1-tubulin C-terminal tail, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
• how: For the motor-pulling assay the authors used a truncated rat kinesin-1 (the first 430 aa of rat Kif5C here denoted as rk430)60 fused with a mScarlet-SNAP-His6 tag at the C-terminus.

SUMMARY

To apply forces to tubulin, the authors developed a strategy to introduce a functional handle . . .

 



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