Genomic insights into the physiology of quinella, an iconic uncultured rumen bacterium

HIGHLIGHTS

• who: Sandeep Kumar from the onto LB agar plates (, g Bacto-tryptone, g yeast extract, g NaCl and , g bacteriological agar dissolved in distilled water made up to , litre, pH, ) containing ampicillin (, u00b5g/mL), bromo-4chloro-3-indolyl-u03b2-D-galactopyranoside (, u00b5g/mL) and isopropyl u03b2-D1-thiogalactopyranoside (, mM) for blue-white colony selection. White colonies were picked as positive and streaked onto LB agar plates containing ampicillin (, u00b5g/mL). Colony PCR was performed to test whether the transformed cells contained a plasmid with the expected insert size. For colony PCR, a colony from a streaked LB . . .
  

   

  

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