HIGHLIGHTS
SUMMARY
The most widely used PDEPR technique is double electron resonance (DEER, or PELDOR), (Milov et_al, 1981; Milov et_al, 1984; Martin et_al, 1998; Schiemann et_al, 2021). Applications of DEER in structural biology rely on pairwise coupling of spinhalf centres with reasonably well-defined distance distributions, especially between nitroxide spin labels and this is the situation that will be discussed in this work (Haugland et_al, 2017; Schiemann et_al, 2021). Probably the most common approach to analyzing DEER time traces with DeerAnalysis is to use Tikhonov regularization with the L-curve method for determining the regularization parameter . . .
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