High-throughput sequencing of ecowi restriction fragments maps the genome-wide landscape of phosphorothioate modification at base resolution

HIGHLIGHTS

• who: Weiwei Yang et al. from the Method and materials Bacteria strains, plasmids and Enzymes The wild-type Salmonella enterica Cerro , strain was kindly provided by DrToshiyki Murase. The gDNA of u0394dndE mutant Salmonella enterica Cerro , was kindly provided by Dr. Xinyi He (mutant gDNA, Shanghai Jiaotong University). The mutant gDNA was purified by a FastPure bacteria DNA isolation kit (Vazyme #DC103). The dnd gene clusters responsible for E. coli PT modification were cloned from Salmonella enterica Cerro , into a pLacZZ (modified from pUC19) backbone and validated as described previously [21]. pLacZZ-dnd+ and the control plasmid . . .
  

   

  

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