HIGHLIGHTS
- who: Christoph Ziegenhain from the Department have published the article: Molecular spikes: a gold standard for single-cell RNA counting, in the Journal: (JOURNAL) of 20/04/2021
- what: The authors demonstrate how molecular spike-ins can be used to rescue faulty experiments or even enable counting in experiments lacking UMIs. The authors show direct experimental evidence that RNA counting based on uncorrected UMIs overestimates RNA expression at a level that follows the chance of PCR and sequencing errors in the UMIs (UMI lengths sequence depth and sequencing technology used). The authors demonstrate that only . . .
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