Transcriptional regulation and chromatin architecture maintenance are decoupled functions at the sox2 locus

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SUMMARY

    Deletion analyses revealed that Sox2 transcription in mouse ESCs and the developing epiblast is regulated by the Sox2 control region (SCR), a 7.3-kb cluster of transcription factor-bound regions located >100 kb downstream from Sox2 (Chen et_al 2012; Li et_al 2014; Zhou et_al 2014; Chakraborty et_al 2022). To identify the sequences required for Sox2 transcription as well as those involved in ESC-specific chromatin topology, the authors used allele-specific CRISPR/Cas9-mediated deletions to systematically remove all transcription factor-bound regions within and surrounding the SCR "superenhancer." The authors found that . . .

     

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