HIGHLIGHTS
- who: Richard S. Ayikpoe from the Chemicals, strains, media Reagents, buffer components, and growth media were purchased from Millipore Sigma (Burlington, MA), Fischer Scientific (Hampton, NH) or Research Products International (Chicago, Illinois) unless otherwise notedIsopropyl u03b2-D-1-thiogalactopyranoside (IPTG), ampicillin and kanamycin were purchased from Gold Biotechnology (Olivette, Missouri). Oligonucleotides, enzymes, and buffers used for molecular biology were purchased from Integrated DNA Technologies Inc. (Coralville, IA) or New England Biolabs (Ipswich, MA). Synthetic genes were purchased from Twist Biosciences (San Francisco, CA). Plasmid isolation was conducted using QIAprep spin columns according to the manufacturer`s protocol . . .
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