HIGHLIGHTS
SUMMARY
Sex chromosomes or the mode of sex determi- nation can vary down to population level in frogs. Prior to performing the sex-linked marker analyses to understand sex-determination in P. ornatum, the authors performed population genetic analyses to infer whether all 44 individuals used in this study belong to a single species. The authors used 4691 autosomal SNP loci (excluding all sex-linked loci) with 0% null allele and 100% reproducibility for the population genetic analyses. In cluster 1, the filtering of loci for sex linkage resulted in 11 SNPs and 24 PA . . .
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