HIGHLIGHTS
SUMMARY
The authors established the novel anti-CD44 mAbs, C44 Mab-5 (IgG1, kappa) and C44 Mab46 (IgG1, kappa) by Cell-Based Immunization and Screening (CBIS) method and immunization of CD44v3-10 ectodomain, respectively. The authors inserted the "RIEDL" peptide into the CD44 protein from the 21st to 41st amino_acids. C44 Mab-5 did not react with the 30th-36th amino_acids of the deletion mutant of CD44. The reaction of C44 Mab-5 to RIEDL tag-inserted CD44 from the 25th to 36th amino_acids was lost, although LpMab-7 detected most of the RIEDL tag . . .
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