S-adenosylmethionine-responsive cystathionine β-synthase modulates sulfur metabolism and redox balance in mycobacterium tuberculosis

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• who: MICROBIOLOGY et al. from the Generation of Mtbu0394cbs knockout and complemented strains One-kilobyte upstream and downstream regions of the genomic locus of cbs (Rv1077) were amplified using , High-Fidelity polymerase and cloned at, u2032 and, u2032 ends, respectively, of the loxP-hyg-gfp-loxP cassette in the mycobacterial sacB-based suicide vector p, (a gift from MNiederweis at the University of Alabama at Birmingham). The complete construct of the flanking sequences and the hyg-gfp cassette was subsequently amplified and cloned into the pRSF-duet vector (Clontech Laboratories, Mountain View, CA, USA) digested with Hpa . . .
  

   

  

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