HIGHLIGHTS
- who: Pascal D. Vos from the Curtin University, Bentley, WA, Australia have published the article: Computationally designed hyperactive Cas9 enzymes, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
- what: The authors engineered the HNH-like nuclease domain of Cas9 (Fig 1) to substantially increase the frequency of donorindependent gene editing through computational design. This model shows that Arg844 may form stabilizing saltbridge interactions with Asp850 (within the HNH domain) and Glu60 (within the Arg domain).
- how: The authors developed a computational design approach called FuncLib that mutates positions within the active_sites of enzymes to . . .
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