Molecular detection of bacterial contamination in plasma using magnetic-based enrichment

HIGHLIGHTS

• who: Jinyeop Lee from the School Sungkyunkwan University have published the research: Molecular detection of bacterial contamination in plasma using magnetic-based enrichment, in the Journal: Scientific Reports Scientific Reports

SUMMARY

Real‑time PCR for the detection of enriched bacteria. DNA was extracted from the enriched Gram- negative and -positive bacteria and subjected to real-time PCR. As shown in Fig 4a, E. coli at ­102 CFU/mL was detected after 12 h of specimen incubation, and Fig 4b shows that E. coli not captured by MNBs were detected at only ­104 CFU . . .

 



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