HIGHLIGHTS
SUMMARY
Pubs.acs.org/acschemicalbiology Articles 1a).15,18 The authors therefore hypothesized that coexpression of an EF-Tu variant that is still active as an elongation_factor, but cannot be phosphorylated by the toxin, could be an effective way to express Doc without affecting cell growth. C-Terminal Phd Peptide Forms a Highly Stable Complex with Doc. Peptide sequences showing high in_vitro inhibitory profiles counteracted Doc toxicity when expressed in S. Typhimurium, demonstrating excellent correlation with in_vitro results. Although most nonhot spot peptides possessed similar Kd values to peptide 1, surprisingly, a significant increase in . . .
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